This approach utilizes consensus primer/adapters to specifically target conserved regions of the 16s ribosomal gene of bacteria for amplifying and sequencing.
- Identification of constituent microbial populations in both clinical and environmental samples
- Identification/tracking of potentially infectious bacteria or sources of bacterial contamination
Omega Bioservices can guide you all the way from project inception to high quality results. Our expert personnel and state-of-the-art equipment allow for high quality data and fast turn-around times. Sequencing is performed on an Illumina MiSeq with V3 chemistry. These chemistries allow you to receive the maximum value for your samples. Results delivered via secure cloud drive or external hard drives are available.
- Fast turn-around time
- Highly experienced personnel
- Data quality analysis report provided
- Customized bioinformatics solutions available
- Dedication to quality
- Quality of data
At Omega Bioservices, we understand that the quality of the DNA going into next generation sequencing is directly related to the quality of the sequence data coming out. That is why quality metrics are captured at vital points during our process:
- Post extraction: DNA is analyzed to ensure it is of sufficient fragment size and quantity for optimal PCR amplification and library preparation chemistries.
- Post library preparation: Fragment size and quantity is again checked to ensure optimal loading onto the Illumina flow cell.
- NGS run: Careful monitoring of the quality (Q) scores along with cluster densities, percentage of reads passing filter, number of reads generated, and average length of reads.
This constant and consistent quality monitoring effort result in the very best product possible delivered to the customer.
As next generation sequencing methodologies continue to improve and evolve, they can generate expansive data sets that address complex biological questions. Existing bioinformatics programs may not be able to adequately address the nuanced aspects of your problem set or simply do not offer the level of analysis that you need.
If you are in need of analysis advice on you NGS data set, or need direct analysis assistance, Omega Bioservices has a network of specialized bioinformatics providers with the expert knowledge and computational resources to help you achieve your data analysis objectives. We can also help in the experimental design at the very beginning of your project to maximize the value of your data. Contact us and let us connect you with the very best bioinformatics capabilities available.
Our understanding and commitment to quality includes our offer to take full control of the quality of your sequence data by offering a complete suite of sample extraction services. Omega Bio-tek has a full product line of manual and automated extraction technologies for most any type of sample that you want to extract and purify both DNA and RNA for next generation sequencing. These sample types include:
- Whole blood, serum, tissue, stool, urine, fungi, bacteria, virus, plant, soil, water, air filter