Soil and Water DNA/RNA Extraction


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Analysis of microfauna and microflora inhabiting soil, water, sediment and sludge is of particular interest in agricultural and environmental research. However, the challenge often lies in the ability to extract DNA or RNA of sufficient quality and quantity from these samples. Soil, for instance, contains high amount of humic substances (humic acids, fulvic acids an humin) which give it a range of color from yellow to dark brown to black. DNA and RNA extracted from soil using a standard kit often inherit this coloration, which interferes with accurate UV quantitation, and more importantly, acts as inhibitor for PCR analysis.

Our soil and water DNA/RNA extraction kits are designed specifically to handle environmental samples. DNA and RNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, and loamy soil samples, water and sewage sludge. Our proprietary color-reducing agents completely removes the coloration, making the isolated DNA and RNA usable for most downstream applications.

In conjunction with the Geno/Grinder and liquid handler robots, we have the capacity of extracting 1,000’s of samples per day.

The Omega Advantage:

  • Proven quality: no coloration, no inhibitors
  • Automated robotic platforms for all extractions
  • NanoDrop quantitation and quality analysis included at no extra charge
  • Free cryostorage and requisition for 1 year

Pricing:

DNA/RNA Extraction Services 1-100 101-500 501-1,000 1,001+
Soil DNA 0.5 g $14 $13 $12 Inquire
Soil DNA 2 g $21 $19 $17 Inquire
Soil RNA 0.5 g $18 $16 $14 Inquire
Soil RNA 2 g $23 $21 $19 Inquire
Water filter DNA $21 $19 $17 Inquire
Water filter RNA $23 $21 $19 Inquire

Turnaround time:

  • Same day service available
  • Standard 3-day
  • Large scale projects, please inquire

Additional Services:

  • Agilent Bioanalyzer QC (RNA)
  • Agarose gel electrophoresis QC (DNA/RNA)
  • Real-time qPCR (DNA/RNA)
  • Sample concentration normalization from tube-to-tube or tube-to-plate (DNA/RNA)
  • Consultation for downstream analyses and study design