FFPE Tissue DNA/RNA Purification


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Formalin-fixed, paraffin-embedded (FFPE) tissue is one of the most widely practiced methods for clinical sample preservation and archiving. While standard for histopathology and microscopic investigation, FFPE tissue samples pose a major challenge for molecular pathologists because nucleic acids are heavily modified and trapped by extensive protein-nucleic acid and protein-protein cross linking. Due to the valuable nature of the FFPE samples, it is paramount that an extraction protocol yields high recovery of DNA/RNA, while also to avoid extensive degradation so that it is amenable for further analysis.

As one of the leading manufacturers for FFPE DNA and RNA extraction kits, we have a complete line of tools for extracting FFPE samples. Validated by numerous third-party laboratories, our kit-based chemistry typically recovers 2-3x more DNA/RNA than the competitor kits.

  • DNA suitable for downstream qPCR, microarray, next generation sequencing
  • Sections, slides, core punches
  • Scalable – Few samples to thousands of samples per day (high throughput automation)
FFPE RNA
Expected Yield: The yield of FFPE samples is highly variable depending on the age, storage condition, fixation method, tissue cellularity and many other factors. We have processed tens of thousands of samples from very different sources, and typically several micrograms of DNA or RNA can be extracted from a 10 micron section. Quite frequently, we can achieve a yield of more than 50 µg.

The Omega Advantage:

  • Ability to process all types of samples: slides, sections, or cores
  • High throughput capacity: FFPE DNA extraction on our liquid handler robotics
  • NanoDrop quantification and quantity analysis included at no extra charge
  • Free cryostorage and requisition for 1 year

Pricing:

FFPE RNA Extraction Service 1-100 101-500 501-1,000 1,001+
FFPE RNA (up to 10 sections) $30 $28 $26 Inquire

Turnaround time:

  • Same day service available
  • Standard 3 day
  • Large sample number and/or starting quantity projects please inquire

Additional Services:

  • Genotyping or mutation analysis
  • Quantitation by real-time qPCR
  • Sample concentration normalization from tube-to-tube or tube-to-plate
  • Consultation for downstream analyses and study design
FFPE DNA
Expected Yield: The yield of FFPE samples is highly variable depending on the age, storage condition, fixation method, tissue cellularity and many other factors. We have processed tens of thousands of samples from very different sources, and typically several micrograms of DNA or RNA can be extracted from a 10 micron section. Quite frequently, we can achieve a yield of more than 50 µg.

The Omega Advantage:

  • Ability to process all types of samples: slides, sections, or cores
  • High throughput capacity: FFPE DNA extraction on our liquid handler robotics
  • NanoDrop quantification and quantity analysis included at no extra charge
  • Free cryostorage and requisition for 1 year

Pricing:

FFPE DNA Extraction Service 1-100 101-500 501-1,000 1,001+
FFPE DNA (up to 10 sections) $30 $28 $26 Inquire

Turnaround time:

  • Same day service available
  • Standard 3 day
  • Large sample number and/or starting quantity projects please inquire

Additional Services:

  • qPCR gene expression analysis
  • Quantitation by real-time qPCR
  • Sample concentration normalization from tube-to-tube or tube-to-plate
  • Consultation for downstream analyses and study design