Region coverage available:
- V1 – V3
- V3 – V4
- V4 – V5
Primer details:
- 27f: 5’-AGAGTTTGATCCTGGCTCAG-3’; Lane et al.
- 27f Modified: 5’-AGMGTTYGATYMTGGCTCAG-3’; Walker et al.
- Illuminaf: 5’-CCTACGGGNGGCWGCAG-3’
- 515f Original: 5′-GTGCCAGCMGCCGCGGTAA-3′, Caporaso et al.
- 515f Modified: 5′-GTGYCAGCMGCCGCGGTAA-3′, Parada et al.
- 534r: 5’-ATTACCGCGGCTGCTGG-3’; Muyzer et al.
- 806r Original: 5’-GGACTACHVGGGTWTCTAAT-3’, Caporaso et al.
- 806r Modified: 5’-GGACTAC NVGGGTWTCTAAT-3’, Apprill et al.
- Illumina R: 5’-GACTACHVGGGTATCTAATCC-3’
- 926r: 5’-CCGYCAATTYMTTTRAGTTT-3’, Parada et al.