Paired-end 300 means that one read of 300 bases in size is generated from each end of the fragment through the inserted middle piece of target DNA from both directions for a total of two (paired) reads per fragment.
Paired-end 300 means that one read of 300 bases in size is generated from each end of the fragment through the inserted middle piece of target DNA from both directions for a total of two (paired) reads per fragment.